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Anigen Canine Heartworm Antigen Test Kit (10 Tests)

Item# A046700
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Description

Anigen Canine Heartworm Antigen Test Kit is an immunochromatographic test for the qualitative detection of canine heartworm antigen (Dirofilaria immitis) in canine serum, plasma, or whole blood. The parasitic worm inhabits the heart of its host and can lead to heart failure in dogs. If your dog is exhibiting symptoms of a heartworm infestation, this test can help make an accurate diagnosis of your pet's condition or eliminate heartworm infestation as a possible cause.

Anigen Canine Heartworm Antigen Test Kit can detect the antigens for these parasites in your pet's feces to determine if an infestation is affecting your pet. This package contains 10 tests and a test kit to determine which virus may be affecting your dog. For more information on what is included in this package and how to use these kits, you can find the full package insert in PDF form here.

Key Benefits

  • Highly purified antibodies produced by biotechnology for the detection and capture of heartworm antigens
  • Highly sensitive and specific
  • Fast results within 5 minutes
  • Simple and easy to use
  • Free confirmatory test for US customers
  • Works with serum, plasma and whole blood
  • Storage at room temperature
  • Small sample volume (40ul)
  • 2-year shelf life

Materials provided(10 tests/kit)

  1. Ten(10) Anigen Rapid CHW Ag test devices
  2. Ten(10) disposable droppers for specimen
  3. Ten(10) Anticoagulant tube
  4. One(1) Instructions for use

Directions

Specimen Collection and Preparation:

  1. The test can be performed with serum, plasma, or whole blood (with anticoagulant such as EDTA). Please follow the below method for specimen collection and Preparation.
  2. If the test kit and specimens are stored refrigerated, they should be brought to room temperature prior to use.

Whole Blood

  1. Blood should be collected with a disposable syringe and added to a tube containing anticoagulant (Heparin, EDTA, or Citrate). For the user's convenienve, Anigen CHW Ag Test Kit 2.0 provides Anrticoagulant tubes containing 20ul of EDTA. Please follow the directions below.
    • A. Put the collected blood sample into the EDTA Tube.
    • B. Close the cap on the EDTA tube and invert the tube five times to mix the blood and the EDTA.
  2. Collected blood should be tested immediately or within 4 hours at room temperature. If blood specimens are not immediately tested, they should be refrigerated at 2-8°C and used within 24 hours.
  3. Severely hemolyzed blood samples may affect the result.

Plasma

  1. Blood should be collected with a disposable syringe and added to a tube containing anticoagulant (Heparin, EDTA, or Citrate), and then separate plasma by centrifugation.
  2. Plasma should be stored at 2-8°C for up to 2 weeks, for longer storage (1 year) freeze at below 20°C.

Serum

  1. Blood should be collected with a disposable syringe and added to a serum collection tube (no anticoagulant).
  2. Collected blood should be left at room temperature for 30 minutes to coagulate, and then separate serum by centrifugation.
  3. Serum should be store at 2-8°C for up to 2 weeks, for longer storage (1 year) freeze at or below -20°.

Procedure of the Test

  1. Remove the test device from the foil pouch, and place it on a flat and dry surface.
  2. Draw up the specimen using the disposable dorpper.
  3. Add two(2) drops (approcimately 80ul) of canine serum, plasma or whole blood into the sample hole. If the migration has not appeared after 1 minute, add one more drop into the sample well.
  4. As the test begins to work, you will see a purple color move across the result window in the center of the test device.
  5. Interpret test results at 5-10 minutes. Do not decide after 20 minutes.

Interpretation of the Test

A colored band "C" will appear in the left section of the result window to show that the test is working properly. This band is the control band. The right section of the result window indicates the test results.

  1. Negative result The presence of only one band "C" within the result window indicates a negative result.
  2. Positive Result The presence of two colored bands ("T" and "C") within the result window, no matter which band appears first indicates a positive result.
  3. Invalid Result If the control purple colored band ("C") is not visible within the result window after performing the test, the result is considered invalid. The directions may not have been followed correctly or the test may have deteriorated. It is recommended that the specimen be re-tested.

Limitations of the test Although the Anigen Rapid CHW Ag Test Kit 2.0 is very accurate in detecting Canine Dirofilaria immitis antigen, a low incidence of false results can occur. Other clinically available tests are required if questionable results are obtained. As with all diagnostic tests, a definitive clinical diagnosis should not be based on the results of a single test, but should only be made by the veterinarian after all clinical and laboratory findings have been evaluated. In a study conducted in the US, the kit showed a specificity of 100% (54/54) and a sensitivity of 96.4% (53/55). In samples with "2 or more female worms" recovered at necropsy, the sensitivity was 100% (41/41). in a separate multicentric field trial study conducted in the US, the sensitivity (15/15) and specificity (5/5) were found to be 100%.

Precautions:

  1. For veterinary diagnostic use only.
  2. For best results, strict adherence to the instructions is required.
  3. All specimens should be handled as being potentially infectious.
  4. Do not open or remove test kits from their individually sealed pouches until immediately before their use.
  5. Do not use the test kit if the pouch is damaged or the seal is broken.
  6. Do not reuse test kits.
  7. All reagents must be at room temperature (15-30°C) before running the assay.
  8. Do not use reagents beyond the stated expiration date marked on the label.
  9. Do not mix components from different lot numbers. The components in this kit have been quality control tested as standard batch unit.

Storage and Stability

The kit can be stored at room temperature (2-30°) or refrigerated. Do NOT FREEZE. Do not store the test kit in direct sunlight. The test kit is stable through the expiration date marked on the package label.

FAQ

We recommend EDTA or heparin for whole blood collection, however you can also use citrate. If results are doubtful, please use serum.
The assay diluent is specific to our test. We do not recommend the use of any other solution as we cannot guarantee that the product will work properly.
A false positive (or negative) result from an individual animal not resulting from technique errors will continue to occur on multiple tests of the same type. Therefore, the false result is usually caused by some unique characteristics of the particular animal, as opposed to a defective test. This characteristic could be a component of the animalís blood, which is able to react in a testís system to show a positive result in the absence of heartworm antigens or a negative result in the presence of heartworm antigens.
  • Animal could have adult worms that die and be left with circulating microfilariae
  • Microfilariae could be Dipetalonema reconditum, a parasite which does not produce the immitis antigen that our test detects.
  • Young dogs born to heartworm-positive mothers could be infected transplacently and be born with circulating microfilariae, but not have adult immitis, therefore no detectable D. immitis antigen.
  • Occasionally, microfilariae will be present before detectable levels of antigen have accumulated.

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